Mechanisms of the White Sea Cod Gadus morhua marisalbi (Gadidae) Intestinal Smooth Muscle Cholinergic Contraction: The Contribution of Various Subtypes of M-cholinergic Receptors and Rho-kinase

Studying the functioning of various body systems in different vertebrates is one key tasks comparative physiology. Teleost fishes are a large group vertebrates; however, mechanisms their digestive tract poorly understood and known mainly for such model object as Danio rerio. The aim this work was to characterize participation subtypes M-cholinergic receptors Rho-kinase enzyme cholinergic contraction smooth muscle intestine White Sea cod Gadus morhua marisalbi (Gadidae). A longitudinal strip cut out from proximal placed device recording contractile responses isometric mode. Contractile reactions response acetylcholine were completely blocked by atropine. blockade M3-cholinergic with 4-DAMP resulted decrease acetylcholine-induced compared control response. M1-receptors pirenzepine also decreased responses, but effect less pronounced receptor blockade. M2-cholinergic methoctramine did not affect magnitude incubation preparations inhibitor accompanied significant well more rapid force after reaching “peak” Thus, causes intestinal activating M3- M1-but receptors. activity contributes development maintenance under action acetylcholine. obtained results interest physiology; they may be important understanding damaging environmental factors on organism teleost fish use preclinical studies drugs.